What question did this study set out to answer?

The aim is to isolate a highly active and stable thrombin preparation from a crude source.

Bovine Thrombin

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Randomized trial examines thrombin purification methods, revealing a stable, active configuration.

The aim is to isolate a highly active and stable thrombin preparation from a crude source.
Fractionation of thrombin using various chromatographic techniques including ion exchange and gel filtration.
Assessment of activity loss over time under different conditions.
Characterization of the molecular weight of isolated thrombin and evaluation of purity.
Stable thrombin preparation achieved with a minimum yield of 91.1% activity.
Thrombin exhibits a specific activity of 1,160 NIH units per absorbance unit post-purification.
Activity loss identified as 0.1% per week at low ionic strength conditions.

Baughman et al. (1967) studied this question.

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