The study demonstrates that AMV reverse transcriptase can synthesize extensive, possibly complete cDNA copies of large polycistronic RNA templates like poliovirus RNA when specific nuclease inhibitors or excess triphosphates are added.
The synthesis of large, possibly complete, complementary DNA (cDNA) copies of poliovirus RNA by avian myeloblastosis virus DNA polymerase is described. The cDNA consists of two size classes, the larger of which is approximately 7500 nucleotides. In the presence of excess deoxynucleoside triphosphates, ribonucleoside triphosphates, or sodium pyrophosphate, only the larger material is obtained. Yields of the large cDNA are 50-75% of the input RNA.
Kacian et al. (Thu,) studied this question.