July 1, 1976Proceedings of the National Academy of SciencesOpen Access

Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.

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Population

Poliovirus (Mahoney Type I) RNA template

Comparison

Avian myeloblastosis virus reverse transcriptase… vs Standard reaction conditions without excess…

Design

Preclinical

Authors

DKDaniel KacianColumbia University JMJeanne C. MyersUniversity of Pennsylvania

Discussion

Member takes

Implication

Supports optimized cDNA synthesis from large RNA; leaves open translation to mammalian or clinical virology applications.

Structured PICO

Population

Poliovirus (Mahoney Type I) RNA template

Intervention

Avian myeloblastosis virus (AMV) reverse transcriptase with excess deoxynucleoside triphosphates, ribonucleoside triphosphates, or sodium pyrophosphate

Comparator

Standard reaction conditions without excess additives

Outcome

Synthesis of large, possibly complete cDNA copies (approximately 7500 nucleotides)

The study demonstrates that AMV reverse transcriptase can synthesize extensive, possibly complete cDNA copies of large polycistronic RNA templates like poliovirus RNA when specific nuclease inhibitors or excess triphosphates are added.

Cite This Study

Kacian et al. (1976) studied this question.

synapsesocial.com/papers/6a204f4812b97f18eeb8355d https://doi.org/10.1073/pnas.73.7.2191

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Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider