Key result
Stabilization of nucleotide-free actin in 48% sucrose permitted the determination of the second-order association rate constant for Mg2+-ATP binding to actin as 5 x 10(6) M-1 s-1.
Sucrose stabilizes nucleotide-free actin, allowing for the measurement of nucleotide binding kinetics, revealing a diffusion-limited reaction.
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Sucrose stabilization enables kinetic assays of nucleotide-free actin; extends quantitative models of cytoskeletal assembly.
Cruz et al. (1995) studied this question. Sucrose vs. dilute buffer was evaluated on Stabilization of nucleotide-free actin and nucleotide binding kinetics. Stabilization of nucleotide-free actin in 48% sucrose permitted the determination of the second-order association rate constant for Mg2+-ATP binding to actin as 5 x 10(6) M-1 s-1.
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