April 1, 1977European Journal of Biochemistry

Association Kinetics and Binding Constants of Nucleoside Triphosphates with G‐Actin

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Population

G-actin and nucleoside triphosphates (1:N6-ethenoadenosine, 5'-triphosphate [xiATP] and ATP) complex

Comparison

Dilution to concentrations between 1 microM and… vs Normal conditions

Design

Preclinical

Authors

FWFelix WaechterSyngenta (Switzerland)JEJürgen EngelRutgers, The State University of New Jersey

Discussion

Member takes

Implication

Challenges prior G-actin affinity estimates; leaves open translation to cardiac cytoskeletal models.

Structured PICO

Population

G-actin and nucleoside triphosphates (1:N6-ethenoadenosine, 5'-triphosphate [xiATP] and ATP) complex

Intervention

Dilution to concentrations between 1 microM and 5 nM; addition of 1 mM EDTA; addition of Ca2+

Comparator

Normal conditions (0.8 mM CaCl2, pH 8.2, 21 degrees C)

Outcome

Dissociation and association rate constants, and binding constants of nucleoside triphosphates with G-actinsurrogate

Direct kinetic measurements reveal that the binding constants of ATP and xiATP to G-actin are 10^3 to 10^4 times higher than previously estimated from indirect data.

Cite This Study

Waechter et al. (1977) studied this question.

synapsesocial.com/papers/6a21c2c7033871db2707c08b https://doi.org/10.1111/j.1432-1033.1977.tb11385.x

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Association Kinetics and Binding Constants of Nucleoside Triphosphates with G‐Actin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Association Kinetics and Binding Constants of Nucleoside Triphosphates with G‐Actin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider