Key result
The first-order rate constant of nucleotide exchange between epsilonATP and ATP in actin strongly depends on ATP and Ca2+ concentrations, with an Arrhenius plot showing a sharp bend near 24 degrees C.
Population
Monomeric actin
Design
Preclinical
Authors
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Actin studies must control for Ca2+ and temperature effects on nucleotide exchange; leaves open relevance to cardiac cytoskeletal dynamics in vivo.
Nucleotide dissociation from monomeric actin is highly dependent on calcium concentration and temperature, with easier dissociation from Ca2+-free actin.
Waechter et al. (1975) studied this question. 1: N6-Ethenoadenosine 5'-triphosphate (epsilonATP) vs. ATP was evaluated on Kinetics of nucleotide exchange. The first-order rate constant of nucleotide exchange between epsilonATP and ATP in actin strongly depends on ATP and Ca2+ concentrations, with an Arrhenius plot showing a sharp bend near 24 degrees C.
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