Key result
The endothelial Ca2+/calmodulin-dependent nitric oxide synthase (ecNOS) isoform is expressed at the mRNA and protein level in human B and T lymphocytes.
This study provides the first evidence that the endothelial ecNOS isoform is expressed at the mRNA and protein level in human lymphocytes.
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ecNOS in lymphocytes identified; leaves open its functional role in immunity and cardiovascular-immune interactions.
Nitric oxide (NO) is a pleiotropic mediator of a variety of cellular processes such as vasorelaxation, neurotransmission, and cytotoxicity. We studied the expression of the human endothelial, Ca2+/calmodulin-dependent NO synthase (NOS) isoform (ecNOS) in highly purified human lymphocytes from peripheral blood and tonsillar T cells. ecNOS mRNA was detected in IgD+ or IgD- B cells, peripheral blood and tonsillar T cells. Upon stimulation, ecNOS mRNA expression decreased in all these lymphocyte subpopulations. Germinal center T cells and follicular dendritic cells did not express ecNOS mRNA either in an unstimulated or in a stimulated state. ecNOS expression by human lymphocytes was further substantiated by its mRNA detection in lymphoid cell lines such as Raji, Daudi, and Jurkat. By the use of a specific monoclonal antibody, ecNOS was shown to be present in T cells from peripheral blood and in various germinal center cells of frozen tonsillar sections. These data are the first to demonstrate the expression of the endothelial ecNOS at mRNA and protein level in human lymphocytes.
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Reiling et al. (1996) studied this question. The endothelial Ca2+/calmodulin-dependent nitric oxide synthase (ecNOS) isoform is expressed at the mRNA and protein level in human B and T lymphocytes.
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