Key result
PCR amplifies the human ecNOS gene with 100% sequence identity in circulating monocytes.
Why the study?
A reliable technique to assess constitutive endothelial nitric oxide synthase (ecNOS) gene expression in human monocytes was needed to monitor ecNOS levels and investigate NO synthesis mechanisms.
The study demonstrates a reliable PCR-based technique for assessing ecNOS mRNA levels in circulating human monocytes, providing a means to investigate mechanisms controlling NO synthase levels.
Facilitates ecNOS mRNA assessment in human monocytes; leaves open clinical translation pending validation studies.
This study evaluates using a polymerase chain reaction (PCR)-based molecular biological approach to study the gene expression of the constitutive endothelial isoform of nitric oxide synthase (ecNOS) in human monocytes. When PCR was carried out with specific primers for ecNOS, 302 bp product was amplified, which sequence analysis determined as having 100% identity to the reported human ecNOS isoform gene sequence. The data presented here demonstrate a reliable technique for assessing ecNOS mRNA levels in circulating human monocytes. This then permits use of this easily available cell to monitor ecNOS levels and provides a means to investigate mechanisms involved in controlling NO synthase levels as well as NO synthesis.
No takes yet. Share an insight, caveat, or question.
Calò et al. (1998) studied this question. PCR-based molecular biological approach was evaluated on Amplification of ecNOS gene product. A PCR-based molecular biological approach successfully amplified a 302 bp product with 100% identity to the human ecNOS isoform gene sequence in circulating human monocytes.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: