July 1, 2003Circulation
Phosphorylation of the I Ks Channel Complex Inhibits Drug Block
Why the study?
Does PKA stimulation inhibit drug block of the IKs channel complex in CHO cells?
Population
CHO cells transfected with KCNQ1 cDNA with or without the ancillary subunit KCNE1
Comparison
Protein kinase A (PKA) stimulation vs Basal IKs (no PKA stimulation)
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Hypothesis-generating for PKA modulation of IKs-drug interactions; leaves open relevance to antiarrhythmic efficacy during sympathetic activation.
Structured PICO
Does PKA stimulation inhibit drug block of the IKs channel complex in CHO cells?
P
Population
CHO cells transfected with KCNQ1 cDNA (encoding the pore-forming subunit) with or without the ancillary subunit KCNE1
I
Intervention
Protein kinase A (PKA) stimulation
C
Comparator
Basal IKs (no PKA stimulation)
O
Outcome
IC50 for quinidine block of IKssurrogate
Activation of the PKA signaling pathway blunts drug block of the IKs channel, identifying a novel mechanism for modulation of drug-channel interactions during β-adrenergic stimulation.
Cite This Study
Yang et al. (2003) studied this question.