August 1, 1992Protein ScienceOpen Access

The accessibility of etheno‐nucleotides to collisional quenchers and the nucleotide cleft in G‐ and F‐actin

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Population

G- and F-actin (in vitro biochemical model)

Comparison

Mg(2+)-induced polymerization and binding of S-1 vs Unpolymerized G-actin or actin without S-1

Design

Preclinical

Authors

Discussion

Hypothesis-generating for actin cleft dynamics; leaves open distinct Mg2+ versus S-1 effects for cardiac validation.

Population

G- and F-actin (in vitro biochemical model)

Intervention

Mg(2+)-induced polymerization and binding of S-1

Comparator

Unpolymerized G-actin or actin without S-1

Outcome

Fluorescence quenching of etheno-nucleotides and accessibility to collisional quencherssurrogate

Mg(2+)-induced polymerization and S-1 binding induce distinct conformational changes in the nucleotide cleft of actin.

Root et al. (1992) studied this question.

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