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We have applied defocused imaging of single fluorescent quantum dots to study the angular distribution of their emission. It is found that quantum dots exhibit an angular distribution best described by a superposition of emission of three perpendicular dipoles. A theory of the defocused images of such emitters is presented and compared with the measurements. Furthermore, it is shown that standard fluorescence anisotropy measurements are not able to uncover such complex emission behavior.
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Patra et al. (2005) studied this question.
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