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This research investigates how connexin and A-CAM antibodies influence the formation of gap and adherens junctions.

Inhibition of gap junction and adherens junction assembly by connexin and A-CAM antibodies.

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This research investigates how connexin and A-CAM antibodies influence the formation of gap and adherens junctions.
Used antibody Fab fragments to alter cell interactions in Novikoff cells.
Conducted immunoblotting and immunocytochemistry to examine protein presence.
Utilized freeze-fracture EM for structural observations of junctions.
Observed no cell-cell dye transfer in 90% of cells treated with connexin or A-CAM antibodies.
Detected gap junctions in 50% of control samples via EM imaging.
Confirmed that extracellular domains of connexins are necessary for gap junction formation.

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Randomized trial demonstrated that connexin and A-CAM antibodies inhibit junction formation, implying a critical role for these proteins.

Meyer et al. (1992) studied this question.

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