What question did this study set out to answer?

To investigate the remodeling of sarcomeric proteins in the mammalian heart using a transgenic model.

March 1, 1996Circulation Research

Transgenic Remodeling of the Contractile Apparatus in the Mammalian Heart

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Population

Adult murine model

Comparison

Expression of a ventricular myosin light chain-2… vs Endogenous expression

Design

Preclinical

Authors

JPJoseph PalermoCincinnati Children's Hospital Medical Center JGJames GulickCincinnati Children's Hospital Medical Center MCMelissa C. ColbertNational Institutes of Health

Discussion

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Implication

Posttranscriptional control dominates sarcomeric stoichiometry in murine atria; leaves open whether transgenic isoform replacement translates to human disease.

Key Points

To investigate the remodeling of sarcomeric proteins in the mammalian heart using a transgenic model.
Expressed a ventricular myosin light chain-2 transgene in atria and ventricles of adult mice using murine alpha-cardiac myosin heavy chain promoter.
Measured transcript levels of transgene and endogenous isoforms in both compartments.
Analyzed the impact of transgene expression on protein levels and stoichiometry of sarcomeric proteins.
Atrium showed complete replacement of atrial myosin light chain-2 isoform by MLC2v, with unchanged transcript levels of endogenous isoform.
Ventricular expression resulted in eightfold increase in MLC2v transcript levels, but no change at the protein level.
Data indicate rigorous maintenance of sarcomeric protein stoichiometry via posttranscriptional regulation.

Structured PICO

Population

Adult murine model

Intervention

Expression of a ventricular myosin light chain-2 transgene (MLC2v) in both atria and ventricles using the murine alpha-cardiac myosin heavy chain gene promoter

Comparator

Endogenous expression

Outcome

Expression levels of MLC2v transcript and protein in atria and ventriclessurrogate

The study demonstrates that sarcomeric protein stoichiometry is rigorously maintained via posttranscriptional regulation and that protein replacement can be achieved through a single transgenic manipulation in a murine model.

Cite This Study

Palermo et al. (1996) studied this question.

synapsesocial.com/papers/6a207b49a40bcbc79e09a0ff https://doi.org/10.1161/01.res.78.3.504

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Implication

Posttranscriptional control dominates sarcomeric stoichiometry in murine atria; leaves open whether transgenic isoform replacement translates to human disease.

Transgenic Remodeling of the Contractile Apparatus in the Mammalian Heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Transgenic Remodeling of the Contractile Apparatus in the Mammalian Heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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