Angiotensin II-induced ventricular hypertrophy in mice resulted in significantly larger intruding angles (P<0.001) and smaller helical angles (P<0.01) of aggregated myocytes compared with controls.
Does ventricular hypertrophy alter the three-dimensional alignment of aggregated myocytes in murine hearts as assessed by DT-MRI?
DT-MRI demonstrates significant alterations in the three-dimensional alignment, specifically helix and intruding angles, of myocytes in hypertrophic murine hearts compared to normal hearts.
p-value: p=<0.001
Several observations suggest that the transmission of myocardial forces is influenced in part by the spatial arrangement of the myocytes aggregated together within ventricular mass. Our aim was to assess, using diffusion tensor magnetic resonance imaging (DT-MRI), any differences in the three-dimensional arrangement of these myocytes in the normal heart compared with the hypertrophic murine myocardium. We induced ventricular hypertrophy in seven mice by infusion of angiotensin II through a subcutaneous pump, with seven other mice serving as controls. DT-MRI of explanted hearts was performed at 3.0 Tesla. We used the primary eigenvector in each voxel to determine the three-dimensional orientation of aggregated myocytes in respect to their helical angles and their transmural courses (intruding angles). Compared with controls, the hypertrophic hearts showed significant increases in myocardial mass and the outer radius of the left ventricular chamber (P < 0.05). In both groups, a significant change was noted from positive intruding angles at the base to negative angles at the ventricular apex (P < 0.01). Compared with controls, the hypertrophied hearts had significantly larger intruding angles of the aggregated myocytes, notably in the apical and basal slices (P < 0.001). In both groups, the helical angles were greatest in midventricular sections, albeit with significantly smaller angles in the mice with hypertrophied myocardium (P < 0.01). The use of DT-MRI revealed significant differences in helix and intruding angles of the myocytes in the mice with hypertrophied myocardium.
Schmitt et al. (2009) studied Ventricular hypertrophy (n=14). Angiotensin II infusion vs. Controls was evaluated on Intruding angles of aggregated myocytes (p=<0.001). Angiotensin II-induced ventricular hypertrophy in mice resulted in significantly larger intruding angles (P<0.001) and smaller helical angles (P<0.01) of aggregated myocytes compared with controls.