Key result
Variation in pH between 5.5 and 7.5 did not significantly alter the conformation of the vinculin tail domain or its weak self-association (Kd approximately 300 microM).
Contrary to previous reports, vinculin tail conformation and self-association appear to be independent of pH changes between 5.5 and 7.5.
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Challenges prior reports of pH-dependent vinculin conformation; leaves open regulatory mechanisms in cellular and in vivo contexts.
Palmer et al. (2008) studied this question. pH variation (pH 5.5 vs pH 7.5) was evaluated on Vt conformation and self-association (K(d)). Variation in pH between 5.5 and 7.5 did not significantly alter the conformation of the vinculin tail domain or its weak self-association (Kd approximately 300 microM).
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