Key result
A minK:CreERT2 BAC transgenic mouse line enables inducible Cre recombination specifically in the AV node, bundle, and bundle branches after tamoxifen administration.
The creation of a minK:CreERT(2) BAC transgenic mouse line enables inducible recombination specifically in the AV node, bundle, and bundle branches, providing a tool to study cardiac conduction system disease and function.
This mouse line enables targeted conduction studies in vivo; leaves open translation to human arrhythmia models.
Inducible Cre recombination is a powerful technology that allows for spatial and temporal modulation of gene expression in vivo. Diseases of the cardiac conduction system (CCS) pose a significant clinical burden but are not currently well understood at the molecular level. To enable inducible recombination in the murine CCS, we created a minK:CreERT(2) bacterial artificial chromosome (BAC) transgenic mouse line. Cre activity is present after tamoxifen administration in the atrioventricular (AV) node, AV bundle, and bundle branches of adult transgenic mice. We anticipate that by enabling inducible recombination specifically in the AV node, bundle, and bundle branches, minK:CreERT(2) BAC transgenic mice will prove useful in advancing our understanding of CCS disease and function.
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Arnolds et al. (2011) studied Diseases of the cardiac conduction system. minK:CreERT2 BAC transgenic mouse line and tamoxifen administration was evaluated on Cre activity in the atrioventricular (AV) node, AV bundle, and bundle branches. A minK:CreERT2 BAC transgenic mouse line enables inducible Cre recombination specifically in the AV node, bundle, and bundle branches after tamoxifen administration.
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