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A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-l-n-hexadecyl)ammonium-2,2,6,6-tetramethylpiperidine-l-oxyl iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer arc primary.
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Wyse et al. (1988) studied this question.
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