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BACKGROUND: The establishment of gut microbiota is a stepwise process contributing to gut development and maturation of the immune system. Aberrant gut microbiota at an early age may predispose to disease later in life. Breast-fed infants harbor a characteristic intestinal microbiota dominated by bifidobacteria. OBJECTIVES: To assess, using molecular techniques (PCR), whether human milk contains bifidobacteria and to determine the bifidobacterial species present. METHODS: The presence of bifidobacteria in breast milk samples (n = 20) was qualitatively and quantitatively determined by using PCR and real-time PCR, respectively. RESULTS: Breast milk samples contained bifidobacteria. Bifidobacterium longum was the most widely found species followed by Bifidobacterium animalis, Bifidobacterium bifidum and Bifidobacterium catenulatum. CONCLUSION: Breast milk contains bifidobacteria and specific Bifidobacterium species that may promote healthy microbiota development.
Gueimonde et al. (Mon,) studied this question.