Key result
Glycine supplementation to peritoneal dialysis solution completely restored the decreased peritoneal microvascular vasoreactivity observed in elderly rats.
Why the study?
Elderly rats showed decreased peritoneal dialysis solution-induced vasoreactivity, hypothesized to be caused by oxidative stress reducing nitric oxide bioavailability that antioxidant glycine might improve.
Does glycine supplementation to peritoneal dialysis solution improve peritoneal microvascular vasoreactivity in elderly rats?
Does glycine supplementation to peritoneal dialysis solution improve peritoneal microvascular vasoreactivity in elderly rats?
Glycine supplementation to peritoneal dialysis solution restores age-related decline in peritoneal microvascular vasoreactivity in rats, suggesting a potential strategy to reduce oxidative stress burden on aged endothelium.
Answers are grounded in this paper and cite their sources.
No practice change for glycine in peritoneal dialysis; leaves open translation from rat data to elderly patients.
Background: Peritoneal dialysis solution (PDS) dilates peritoneal microvessels predominantly by the activation of the endothelial nitric oxide (NO) pathway. We made an incidental observation of decreased PDS-induced, NO-dependent peritoneal microvascular vasoreactivity in elderly rats naïve to PDS exposure. We hypothesized that this subordinate NO-mediated peritoneal microvascular vasoreactivity is caused by increased oxidative stress in the aged endothelium, which compromises NO bioavailability in the elderly, and that peritoneal microvascular vasoreactivity can be improved by the supplementation of antioxidant glycine to PDS. Methods: We studied PDS-mediated vasoreactivity of four intestinal visceral arterioles of different orders by in vivo intravital microscopy in weaned, adult, and elderly rats to (i) confirm subordinate vasoreactivity to PDS in elderly rats; (ii) restore vasoreactivity by glycine supplementation; and (iii) establish age as an independent risk factor for endothelial cell dysfunction. Results: In a crossover series, peritoneal microvascular vasoreactivity to PDS exposure was remarkably decreased in elderly rats. This subordinate vasoreactivity was completely restored by the supplementation of glycine to PDS. In a separate series, we assessed in situ endothelial cell function in weaned and adult rats using the cumulative acetylcholine concentration–response curves. Unlike the adults, the weaned rats demonstrated remarkable sensitivity and reactivity to cumulative acetylcholine concentrations, suggesting the dependency of endothelial cell function on age. Conclusion: Aging is an independent risk factor for peritoneal microvascular endothelial cell dysfunction. Endothelial function in the elderly can be recovered by reinforcing the bioavailability of endothelial-derived NO through glycine. Dietary glycine supplementation is a potential therapeutic strategy to decrease the burden of oxidative stress on the aged endothelium.
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Zakaria et al. (2019) studied Peritoneal microvascular endothelial cell dysfunction. Glycine supplementation to peritoneal dialysis solution vs. Peritoneal dialysis solution without glycine was evaluated on Peritoneal microvascular vasoreactivity. Glycine supplementation to peritoneal dialysis solution completely restored the decreased peritoneal microvascular vasoreactivity observed in elderly rats.
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