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Automated continuous-flow procedures have been developed for microdetermination of immunoglobulins IgG, IgA, and IgM in human serum. The three immunoglobulins are measured by reactions with specific commercial antiserum, and standardized by use of pooled human serum containing known concentrations of IgG, IgA, and IgM. Values determined for the immunoglobulins by the automated procedures correlate with values obtained by radial immunodiffusion. The precision of replicate determinations for the IgG, IgA, and IgM methods is 3.64, 5.11, and 6.00% (CV), respectively.
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Killingsworth et al. (1971) studied this question.
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