Systemic and mucosal immune responses following oral adenoviral delivery of influenza vaccine to the human intestine by radio controlled capsule

Figures

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  Figure 1. Scintigraphy visualization of subjects given vaccine released in the upper small intestine (A) versus the lower small intestine (B). Each subject swallows vaccine in a size 000 mechanical capsule loaded with liquid vaccine and a radiolabeled tracer. Post dose, one can visualize the capsule transiting through the stomach (Stom, red circle) and the intestine as a discrete spot because the radiolabeled tracer is all contained with the capsule. As the radiolabeled tracer proceeds through the proximal small intestine (PSI, purple circle) and the distal small intestine (DSI, yellow circle), the figures are labeled with such. Post release, the liquid contents are ejected from the capsule and dispersion of the material can be visualized as it spreads away from the capsule. When verified that the dispersed material has reached the colon (Colon, green outlined region), scintigraphy visualization is no longer required.

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  Figure 2. IgA and IgG ASC responses to HA following rAd oral vaccination to either the jejunum or ileum. Results are shown as numbers of HA-specific ASCs/106 PBMC 7 days after vaccination. Each icon represents response level of one subject (A,B). Each bar represents median.

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  Figure 3. Antibody responses to HA following rAd oral vaccination to either the jejunum or ileum. (A) HAI titers to A/CA/07/2009 on days 0 and 28 after a single dose for the subjects that were not seroprotected (initial HAI < 40) at the start of the study. The line on the figure indicates a titer of 40 to show subjects seroprotected post immunization. (B) MN titers to A/CA/07/2009 for individual subjects on days 0 and 28 post immunization. The line shows the MN titer of 40.

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  Figure 4. Fold increases in the anti-HA IgA responses to influenza HA following vaccine delivery by RCC to either the jejunum or ileum. Data are shown when a > 2-fold increase in GMT from day 0 to day 28 nasal and fecal samples had occurred.

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  Figure 5. (A) The proportion of β7(high) as a percentage of CD19+B cells in PBMCs are shown days 0 and 7 following vaccination in either the jejunum or ileum (**p = 0.0024 for the jejunum-targeted group and p = 0.0034 for the ileum-targeted group at day0 and day7, Wilcoxon test of paired t test). Each icon represents an individual subject. Bars represent the median of β7(high) CD19+B cells. The red arrow indicates a subject that was analyzed for FACS data in Figs 5 and 6. (B) Samples of CD19 versus β7 staining are shown on day 0 and day 7 PBMCs. (C) Both β7(intermediate) and β7(negative) CD19+B cells expressed α4 integrin (blue line). The negative control (FMO) is shown in orange. CD19+β7(high) cells have high expression of α4 integrin (red line). (D) On day7 following vaccination, β7(high) B cells in PBMCs express CD27(high) and CD27(lintermediate). (E) Gating on β7(high) and β7(negative) B cells, surface IgA and IgG expression are shown. (F) β7(high) IgA + B cells show CCR9 expression compared to β7(negative) B cells. Population 1: day 7 β7(high) CD19+B cells, population 2: day 7 β7(intermediate) CD19+B cells, population 3: β7(negative) CD19+B cells.

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  Figure 6. (A) After vaccination, CD27(high) B cells appear in PBMCs. (B) CD27(high) B cells express high levels of CD38 (red line) compared to CD27(negative) (blue line). (C) The gated cells on day7 in (A) are shown for their FSC and SSC profile. CD27(hi) cells are larger than CD27(intermediate) B cells. (D) β7 expression on CD27(high) B cells. The major sub population expressed β7(high). (E) β1 integrin versus β7 integrin expression gated on CD27(high) B cells. (F) IgA and IgG surface expressions gated on CD27(high) B cells on day7. (G,H) comparison of intestinal and non-intestinal mucosal B cells in jejunum and ileum release groups.

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  Figure 7. A schematic representation of the subsets generated after rAd oral vaccination. Population 1: day 7 CD27(high)CD19+B cells, population 2: day 7 CD27(intermediate) CD19+B cells, population 3: CD27(negative) CD19+B cells, population 4: CD27(high)CD19+β7(high) β1+, population 5, CD27(high)CD19+β7(intermediate) β1+, population 6: CD27(high)CD19+β7(negative) β1+.