Why the study?
Does heavy meromyosin exist in a refractory state unable to bind to actin in the presence of ATP?
Population
In vitro biochemical model using heavy meromyosin (HMM) prepared from myosin and actin
Comparison
Addition of ATP vs Absence of ATP or presence of ADP, EDTA, or Ca
Design
Preclinical
Authors
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Hypothesis-generating for myosin kinetics in sarcomere function; leaves open validation in human cardiac muscle.
Does heavy meromyosin exist in a refractory state unable to bind to actin in the presence of ATP?
Kinetic and ultracentrifuge studies suggest that heavy meromyosin exists in a refractory state unable to bind to actin during a large part of its cycle of interaction with actin and ATP.
Eisenberg et al. (1972) studied this question.
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