Key result
Mechanical strain altered IGF-I gene splicing in C2C12 cells, with a single ramp stretch upregulating both IGF-IEa and MGF, whereas cyclical stretch reduced IGF-IEa but upregulated MGF.
Why the study?
Does mechanical strain influence IGF-I gene splicing in developing muscle cells?
Does mechanical strain influence IGF-I gene splicing in developing muscle cells?
Mechanical strain differentially regulates the splicing of the IGF-I gene into IGF-IEa and MGF in developing skeletal muscle cells.
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Strain-specific IGF-I splicing in myoblasts should not yet inform loading protocols; leaves open translation to in vivo muscle adaptation.
Cheema et al. (2004) studied Skeletal muscle development. Mechanical strain (single ramp stretch or repeated cyclical stretch) vs. Static cultures was evaluated on Expression of IGF-IEa and MGF splice variants. Mechanical strain altered IGF-I gene splicing in C2C12 cells, with a single ramp stretch upregulating both IGF-IEa and MGF, whereas cyclical stretch reduced IGF-IEa but upregulated MGF.
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