Why the study?
Whether RPS3 mRNA-binding contributes to specific mRNA translation and ribosome specialization in mammalian cells was unknown.
Does mutation of RPS3 mRNA-contacting residues affect cellular and viral (SARS-CoV-2) translation in mammalian cells?
Population
Mammalian cells
Comparison
RPS3 mRNA-contacting residue mutations (R116, R146, K148)
Design
In vitro experimental study
Key result
Mutation of RPS3 mRNA-binding residues altered start-codon fidelity and scanning, and impaired SARS-CoV-2 NSP1-mediated inhibition of host translation and viral mRNA degradation.
Authors
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RPS3 residues may represent a host target to counter NSP1 effects; leaves open whether this extends to antiviral strategies in humans.
Does mutation of RPS3 mRNA-contacting residues affect cellular and viral (SARS-CoV-2) translation in mammalian cells?
RPS3 mRNA-binding residues play crucial roles in regulating host translation and are exploited by SARS-CoV-2 to influence viral and host mRNA translation and stability.
Havkin-Solomon et al. (2023) studied SARS-CoV-2 infection / mRNA translation. Mutation of RPS3 mRNA-contacting residues (R116, R146, K148) vs. Wild-type RPS3 was evaluated on Impact on cellular and viral translation. Mutation of RPS3 mRNA-binding residues altered start-codon fidelity and scanning, and impaired SARS-CoV-2 NSP1-mediated inhibition of host translation and viral mRNA degradation.