Key result
Targeted ultrasound imaging using annexin A5 microbubbles successfully identified early apoptosis in rats with acute doxorubicin-induced cardiomyopathy.
Why the study?
Does targeted ultrasound imaging with annexin A5 microbubbles detect early apoptosis in a rat model of acute doxorubicin-induced cardiotoxicity?
Does targeted ultrasound imaging with annexin A5 microbubbles detect early apoptosis in a rat model of acute doxorubicin-induced cardiotoxicity?
Targeted ultrasound imaging using annexin A5 microbubbles can noninvasively detect early apoptosis in a rat model of acute doxorubicin-induced cardiomyopathy before left ventricular systolic dysfunction occurs.
Answers are grounded in this paper and cite their sources.
May enable preclinical cardiotoxicity monitoring; leaves open human translation and validation.
BACKGROUND: The aim of this study was to assess the feasibility of targeted ultrasound imaging on apoptosis with annexin A5 microbubbles (A5MB) in acute doxorubicin-induced cardiotoxicity. METHODS: Avidinated and octafluoropropan-filled phospholipid microbubbles were conjugated with biotinylated annexin A5. To confirm the specific binding of A5MB, flow cytometry was performed with hydrogen peroxide induced apoptosis in rat aorta smooth muscle cells incubated with fluorescein-5-isothiocyanate (FITC) labeled annexin A5 and A5MB. Adult male rats were injected intraperitoneally with 5 mg/kg doxorubicin weekly for 3 weeks (n = 5). Control rats were injected with normal saline (n = 5). At 24 hours after the final treatment, triggering imaging was performed 15 min after an intravenous bolus injection of A5MB for washout of freely circulating microbubbles. After echocardiography, the heart was isolated for histological detection of apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. RESULTS: In the in vitro tests, fluorescence intensity was low for healthy cells and high for apoptotic cells when incubated with FITC-labeled annexin A5 and A5MB. Rats treated with doxorubicin showed significant contrast opacification of the myocardium on contrast echocardiography using A5MB. However, no opacification was observed in control rats. Apoptosis was confirmed by TUNEL assay in doxorubicin treated rats. CONCLUSION: Acute doxorubicin-induced cardiomyopathy based on early apoptosis can be assessed and imaged with targeted ultrasound imaging using A5MB in rats.
No takes yet. Share an insight, caveat, or question.
Min et al. (2010) studied Acute doxorubicin-induced cardiotoxicity (n=10). Targeted ultrasound imaging with annexin A5 microbubbles vs. Control rats (normal saline) was evaluated on Contrast opacification of the myocardium on echocardiography. Targeted ultrasound imaging using annexin A5 microbubbles successfully identified early apoptosis in rats with acute doxorubicin-induced cardiomyopathy.
Synapse has enriched 10 related papers. Consider them for comparative context: