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Superparamagnetic nanosensors conjugated to avidin (CLIO-A) can be used to measure sensitively and quantitively the proteolytic cleavage of bi-biotinylated peptide substrates (rods in the scheme) by changes in magnetic resonance properties. This approach may be generalized to determine the activity of a wide range of hydrolytic enzymes.
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Zhao et al. (2003) studied this question.
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