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Combining a simple chromatographic system on celite microcolumns with partially specific antisera as binding reagents, simultaneous radioimmunoassay of progesterone, 17-hydroxyprogesterone, and estradiol-17B could be performed on the same aliquot of plasma. These steroids have been measured in plasma samples obtained from subjects of both sexes under various conditions.
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Abraham et al. (1971) studied this question.
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