Randomized trial identifies amyloid precursor-like protein in mouse brain, suggesting its role in Alzheimer disease.
Key Points
The research aims to isolate and characterize a cDNA that encodes a protein related to the amyloid beta protein precursor, exploring its implications in Alzheimer disease.
Isolated cDNA from mouse brain library encoding a 653-amino acid protein.
Conducted hybridization to assess mRNA presence in mouse brain and neuroblastoma cells.
Utilized polyclonal antibodies for protein localization studies.
Identified APLP with 42% identity and 64% similarity to APP.
APLP shows significant homology with both Drosophila and rat APP-like proteins.
Demonstrated hybridization signals for two mRNAs, approximately 2.4 and 1.6 kilobases, in relevant tissues.