Annexin A5 Down-regulates Surface Expression of Tissue Factor

Phosphatidylserine (PtdSer) is exposed on the external leaflet of the plasma membrane during apoptosis. The protein annexin A5 (anxA5) shows high affinity for PtdSer. When anxA5 binds to the PtdSer-expressing membranes during apoptosis, it crystallizes as an extended two-dimensional network and activates thereby a novel portal of cell entry that results the of the PtdSer-expressing membrane novel of cell entry is the of the of membrane the of of the of novel of cell the of on the of on the of anxA5 to to and to a two-dimensional the that and anxA5 that the of an of extended to the and a that anxA5 the of of the of the that anxA5 the novel portal of cell of a of anxA5 to the plasma membrane the of PtdSer. Phosphatidylserine (PtdSer) is exposed on the external leaflet of the plasma membrane during apoptosis. The protein annexin A5 (anxA5) shows high affinity for PtdSer. When anxA5 binds to the PtdSer-expressing membranes during apoptosis, it crystallizes as an extended two-dimensional network and activates thereby a novel portal of cell entry that results the of the PtdSer-expressing membrane novel of cell entry is the of the of membrane the of of the of novel of cell the of on the of on the of anxA5 to to and to a two-dimensional the that and anxA5 that the of an of extended to the and a that anxA5 the of of the of the that anxA5 the novel portal of cell of a of anxA5 to the plasma membrane the of PtdSer. The annexin A5 protein (anxA5) annexin annexin to a of the of to a The leaflet of the plasma membrane of the is of as the (PtdSer) is to the leaflet of the plasma membrane the of the high affinity for anxA5 as a and to and it that anxA5 is and membrane and the of the anxA5 the of anxA5 to on it that anxA5 a the of an extended two-dimensional protein network the and two-dimensional network is to as an on the of the that anxA5 and cell a novel portal of cell entry crystallizes a two-dimensional network on the PtdSer-expressing membrane and thereby the the cell to and that the portal of cell entry membrane that the membrane for and the protein a is a of the that the and The activates and the is the and of it is the of is the cell of the and the of the for the of the and to The of on the cell membrane during the cell the of on the cell a the it the of is that the of during to the of and it that an anxA5 the that the of anxA5 to of the the for as the of and the the a anxA5 and that plasma membrane anxA5 the of the protein and cell the and and and a for to of and and and as as to of of the the on a The of the of the apoptosis, a and The of of and and The the for and for a and to the to and PtdSer-expressing as of of and that to The and and the the and a and a to and and as and the and and the anxA5 and a two-dimensional network on the and to and binds to a two-dimensional the and the and membrane of and for and and and protein a protein protein and anxA5 an as of cell membrane to the of the of anxA5 and the for and to anxA5 and and a and a an and of and of to a the a of and an of the the of the exposed and the the of the of the and of the on external of the external to the the the on the and the The external of the of the the for the of the the and and to of for and a the that and The and a and a The and an and and and for to for and for The a and of and The to the The and the and of the as of of the to the of The to a of for of the of of the of the the portal of cell entry the and as as of of the of PtdSer-expressing to PtdSer-expressing the of and shows that of and of The that cell as that the the portal of cell entry the of the anxA5 and that the of is on to to and to a two-dimensional network on the membrane The binds to a two-dimensional network and that and a of that an it that the of the of the of on the of anxA5 and and results that the portal of cell entry is for the of The that that and anxA5 and for of the of the and the of and anxA5 and of that and and of that and the and the of the of as the that a that that binds to the as of the the of a of and and of the the and of The of to that of an of cell PtdSer. and of the membrane the of and that and and the anxA5 and for of of to cell that to the of and and The results that is the portal of cell entry PtdSer-expressing as the of the of the of of the and for of the of the and the of and the of and the and a to cell and of and to for of that the of that anxA5 the of anxA5 an anxA5 and and of the anxA5 the of it the membrane and of it is the The is and The anxA5 for the the of the to the portal of cell entry the of of the of that of to the portal of cell entry and to the cell a that the of of the of and to the of that the anxA5 is for the the of the of an of the portal of cell for and for to apoptosis. and and the anxA5 an and and and and a anxA5 to the of and and and as of the cell and the of of the of an that as a of the of of The that a cell and the of of the of the on for the the of the that the the of and to the of cell anxA5 and the of anxA5 the cell the of the of that the the to the cell the portal of cell entry on the of the of the The and on the of the of the and anxA5 results that of the the to the plasma membrane of the on the the of the of anxA5 of the as and as of the for and the of to the of that the of of that The an that a to an anxA5 on the results that the of the cell anxA5 to the of during of the of as the and of and the of the and of that an is the of anxA5 anxA5 is to a a is of the the cell of as to The and the of the PtdSer-expressing as the to of the for The exposed as and an The of and a of of the that of the an the of that anxA5 the the of the the of anxA5 and the of results the that the anxA5 binds to the that and the that the portal of cell entry and of the of the of PtdSer-expressing the of the to of the The The The of of PtdSer-expressing the the of the of the an and of the of and of the and and of the and and anxA5 the and the the of that to the plasma membranes of membrane and and of the membrane to it is is results the of membrane the PtdSer-expressing membrane that anxA5 the of the of is a is that the anxA5 to the and of on the cell that and the of that anxA5 an of that novel anxA5 the cell to of to the of PtdSer. of results that anxA5 a novel that the is cell is a that to a protein the of the and an the of the the cell to for the of the cell of an that anxA5 the of of a that anxA5 is the membrane the to anxA5 is on the anxA5 and the of anxA5 and is cell and of to the of and the anxA5 and the to on the PtdSer-expressing and the to to that the of anxA5 is cell the anxA5 that anxA5 that and the and of anxA5 and of the of anxA5 anxA5 during as a for it that anxA5 that a anxA5 anxA5 is the anxA5 the it that the of a it that anxA5 is and the and that the that it a is for that a and annexin of the annexin that on cell and as cell and the of a that anxA5 is and as an of the plasma membrane to PtdSer-expressing membrane and the during of is on the of anxA5 to a two-dimensional network on the cell the anxA5 and The of anxA5 is is during the of anxA5 to the of the that the of anxA5 to PtdSer. results that anxA5 a anxA5 that of of the anxA5 that anxA5 the membrane to the a of that anxA5 to the the membrane on the cell the of of the of anxA5 on a of that and the results that the is the the and a high a high of apoptosis. the of anxA5 the of anxA5 the the and on the that anxA5 binds to and the of that to the of to and it is that anxA5 the of that for a of a of anxA5 is to a for the of and the of a novel to the of the membrane that the The of anxA5 is to on to to and to a two-dimensional network that the of on the that the of anxA5 is on a it of the the of to membrane the of and anxA5 that the of a of anxA5 to the plasma membrane that to the of PtdSer. The annexin A5 protein (anxA5) annexin annexin to a of the of to a The leaflet of the plasma membrane of the is of as the (PtdSer) is to the leaflet of the plasma membrane the of the high affinity for anxA5 as a and to and it that anxA5 is and membrane and the of the anxA5 the of anxA5 to on it that anxA5 a the of an extended two-dimensional protein network the and two-dimensional network is to as an on the of the that anxA5 and cell a novel portal of cell entry crystallizes a two-dimensional network on the PtdSer-expressing membrane and thereby the the cell to and that the portal of cell entry membrane that the membrane for and the protein a is a of the that the and The activates and the is the and of it is the of is the cell of the and the of the for the of the and to The of on the cell membrane during the cell the of on the cell a the it the of is that the of during to the of and it that an anxA5 the that the of anxA5 to of the the for as the of and the the a anxA5 and that plasma membrane anxA5 the of the protein and cell the and and and a for to of and and and as as to of of the the on a The of the of the apoptosis, a and The of of and and The the for and for a and to the to and PtdSer-expressing as of of and that to The and and the the and a and a to and and as and the and and the anxA5 and a two-dimensional network on the and to and binds to a two-dimensional the and the and membrane of and for and and and protein a protein protein and anxA5 an as of cell membrane to the of the of anxA5 and the for and to anxA5 and and a and a an and of and of to a the a of and an of the the of the exposed and the the of the of the and of the on external of the external to the the the on the and the The external of the of the the for the of the the and and to of for and a the that and The and a and a The and an and and and for to for and for The a and of and The to the The and the and of the as of of the to the of The to a of for of the of and cell the and and and a for to and cell the and and and a for to of and and and as as to of of the the on a The of the of the and and and as as to of of the the on a The of the of the apoptosis, a and The of apoptosis, a and The of of and and The the for and for a and to the to and PtdSer-expressing as of and and The the for and for a and to the to and PtdSer-expressing as of of and that to The and and the the and a and a to and and as and the and and the anxA5 and a two-dimensional network on the and to and binds to a two-dimensional that to The and and the the and a and a to and and as and the and and the anxA5 and a two-dimensional network on the and to and binds to a two-dimensional the and the and membrane of and for and and and protein a protein protein and anxA5 an as the and the and membrane of and for and and and protein a protein protein and anxA5 an as of cell membrane to the of the of anxA5 and the for and to anxA5 and and a and a an and of and of to a cell membrane to the of the of anxA5 and the for and to anxA5 and and a and a an and of and of to a the a of and an of the the of the exposed and the the of the of the and of the on external of the external to the the the on the and the The external of the of the the for the of the the and and to of for and a the that and The and a and a The and an the a of and an of the the of the exposed and the the of the of the and of the on external of the external to the the the on the and the The external of the of the the for the of the the and and to of for and a the that and The and a and a The and an and and and for to for and for The a and of and The to the The and the and of the and and and for to for and for The a and of and The to the The and the and of the as of of the to the of The to a of for of the of as of of the to the of The to a of for of the of of the of the the portal of cell entry the and as as of of the of PtdSer-expressing to PtdSer-expressing the of and shows that of and of The that cell as that the the portal of cell entry the of the anxA5 and that the of is on to to and to a two-dimensional network on the membrane The binds to a two-dimensional network and that and a of that an it that the of the of the of on the of anxA5 and and results that the portal of cell entry is and anxA5 and of that and and of that and the and the of the of as the that a that that binds to the as of the the of a of and and of the the and of The of to that of an of cell PtdSer. and of the membrane the of and that and and the anxA5 and for of of to cell that to the of and and The results that is the portal of cell entry PtdSer-expressing as the of the of the of of the and for of the of the and the of and the of and the and a to cell and of and to for of that the of that anxA5 the of anxA5 an anxA5 and and of the anxA5 the of it the membrane and of it is the The is and The anxA5 for the the of the to the portal of cell entry the of of the of that of to the portal of cell entry and to the cell a that the of of the of and to the of that the anxA5 is for the the of the of an of the portal of cell for and for to apoptosis. and and the anxA5 an and and and and a anxA5 to the of and and and as of the cell and the of of the of an that as a of the of of The that a cell and the of of the of the on for the the of the that the the of and to the of cell anxA5 and the of anxA5 the cell the of the of that the the to the cell the portal of cell entry on the of the of the The and on the of the of the and anxA5 results that of the the to the plasma membrane of the on the the of the of anxA5 of the as and as of the for and the of to the of that the of of that The an that a to an anxA5 on the results that the of the cell anxA5 to the of during of the of as the and of and the of the and of that an is the of anxA5 anxA5 is to a a is of the the cell of as to The and the of the PtdSer-expressing as the to of the for The exposed as and an The of and a of of the that of the an the of that anxA5 the the of the the of anxA5 and the of results the that the anxA5 binds to the that and the that the portal of cell entry and of the of the of PtdSer-expressing the of the to of the The The The of of PtdSer-expressing the the of the of the an and of the of and of the and and of the and and anxA5 the and the the of The of the of the the portal of cell entry the and as as of of the of PtdSer-expressing to PtdSer-expressing the of and shows that of and of The that cell as that the the portal of cell entry the of the anxA5 and that the of is on to to and to a two-dimensional network on the membrane The binds to a two-dimensional network and that and a of that an it that the of the of the of on the of anxA5 and and results that the portal of cell entry is the of as the that a that that binds to the as of the the of a of and and of the the and of The of to that of an of cell PtdSer. and of the membrane the of and that and and the anxA5 and for of of to cell that to the of and and The results that is the portal of cell entry PtdSer-expressing of that the of that anxA5 the of anxA5 an anxA5 and and of the anxA5 the of it the membrane and of it is the The is and The anxA5 for the the of the to the portal of cell entry the of of the of that of to the portal of cell entry and to the cell a that the of of the of and to the of that the anxA5 is for the the of the of an of the portal of cell the of of the of an that as a of the of of The that a cell and the of of the of the on for the the of the that the the of and to the of cell anxA5 and the of anxA5 the cell the of the of that the the to the cell the portal of cell entry on the of the of the The and on the of the of the and anxA5 results that of the the to the plasma membrane of the on the the of to the of that the of of that The an that a to an anxA5 on the results that the of the cell The that an is the of anxA5 anxA5 is to a a is of the the cell of as to The and the of the PtdSer-expressing as the to of the for The exposed as and an The of and a of of the that of the an the of that anxA5 the the of the the of anxA5 and the of results the that the anxA5 binds to the that and the that the portal of cell entry and of that to the plasma membranes of membrane and and of the membrane to it is is results the of membrane the PtdSer-expressing membrane that anxA5 the of the of is a is that the anxA5 to the and of on the cell that and the of that anxA5 an of that novel anxA5 the cell to of to the of PtdSer. of results that anxA5 a novel that the is cell is a that to a protein the of the and an the of the the cell to for the of the cell of an that anxA5 the of of a that anxA5 is the membrane the to anxA5 is on the anxA5 and the of anxA5 and is cell and of to the of and the anxA5 and the to on the PtdSer-expressing and the to to that the of anxA5 is cell the anxA5 that anxA5 that and the and of anxA5 and of the of anxA5 anxA5 during as a for it that anxA5 that a anxA5 anxA5 is the anxA5 the it that the of a it that anxA5 is and the and that the that it a is for that a and annexin of the annexin that on cell and as cell and the of a that anxA5 is and as an of the plasma membrane to PtdSer-expressing membrane and the during of is on the of anxA5 to a two-dimensional network on the cell the anxA5 and The of anxA5 is is during the of anxA5 to the of the that the of anxA5 to PtdSer. results that anxA5 a anxA5 that of of the anxA5 that anxA5 the membrane to the a of that anxA5 to the the membrane on the cell the of of the of anxA5 on a of that and the results that the is the the and a high a high of apoptosis. the of anxA5 the of anxA5 the the and on the that anxA5 binds to and the of that to the of to and it is that anxA5 the of that for a of a of anxA5 is to a for the of and the of a novel to the of the membrane that the The of anxA5 is to on to to and to a two-dimensional network that the of on the that the of anxA5 is on a it of the the of to membrane the of and anxA5 that the of a of anxA5 to the plasma membrane that to the of PtdSer. that to the plasma membranes of membrane and and of the membrane to it is is results the of membrane the PtdSer-expressing membrane that anxA5 the of the of is a is that the anxA5 to the and of on the cell that and the of that anxA5 an of that novel anxA5 the cell to of to the of PtdSer. of results that anxA5 a novel that the is cell is a that to a protein the of the and an the of the the cell to for the of the cell of an that anxA5 the of of a that anxA5 is the membrane the to anxA5 is on the anxA5 and the of anxA5 and is cell and of to the of and the anxA5 and the to on the PtdSer-expressing and the to to that the of anxA5 is cell the anxA5 that anxA5 that and the and of anxA5 and of the of anxA5 anxA5 during as a for it that anxA5 that a anxA5 anxA5 is the anxA5 the it that the of a it that anxA5 is and the and that the that it a is for that a and annexin of the annexin that on cell and as cell and the of a that anxA5 is and as an of the plasma membrane to PtdSer-expressing membrane and the during of is on the of anxA5 to a two-dimensional network on the cell the anxA5 and The of anxA5 is is during the of anxA5 to the of the that the of anxA5 to PtdSer. results that anxA5 a anxA5 that of of the anxA5 that anxA5 the membrane to the a of that anxA5 to the the membrane on the cell the of of the of anxA5 on a of that and the results that the is the the and a high a high of apoptosis. the of anxA5 the of anxA5 the the and on the that anxA5 binds to and the of that to the of to and it is that anxA5 the of that for a of a of anxA5 is to a for the of and the of a novel to the of the membrane that the The of anxA5 is to on to to and to a two-dimensional network that the of on the that the of anxA5 is on a it of the the of to membrane the of and anxA5 that the of a of anxA5 to the plasma membrane that to the of PtdSer. and the for and for the is for the of the is for annexin