Key result
Myosin V step size was proportional to neck length, and a neck containing more than two calmodulin binding motifs was necessary for robust processive movement on actin.
Population
Recombinant myosin V heavy meromyosin-like fragments containing 6IQ motifs and mutants with shortened…
Comparison
Alteration of neck length by changing the number… vs Wild-type myosin V heavy meromyosin-like…
Design
Preclinical
Authors
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Extends lever-arm model for myosin V; leaves open relevance to cardiac myosins or contractility.
The extended neck length of myosin V is critical for its processive movement and determines its step size along actin filaments.
Sakamoto et al. (2003) studied this question. Myosin V heavy meromyosin-like fragments with altered neck lengths (2IQ, 4IQ, 6IQ, 8IQ) vs. Wild type (6IQ) or other neck lengths was evaluated on Step size and processivity on actin filaments. Myosin V step size was proportional to neck length, and a neck containing more than two calmodulin binding motifs was necessary for robust processive movement on actin.
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