Randomized trial demonstrates the role of IuMYB114 in regulating anthocyanin biosynthesis in Impatiens uliginosa, suggesting implications for ornamental traits.
Key Points
This research aims to explore the molecular mechanisms underlying petal variegation in Impatiens uliginosa, focusing on the role of specific genes.
Used morphological and molecular approaches to analyze petal regions of Impatiens uliginosa.
Conducted RNA-seq analysis to identify candidate genes involved in variegation.
Applied virus-induced gene silencing (VIGS) to validate the function of the IuMYB114 gene.
Variegated dorsal petals showed three times more pigment-accumulating cells compared to non-variegated regions.
Silencing IuMYB114 resulted in an 89% reduction in expression and decreased pigmentation intensity.
Key anthocyanin biosynthetic genes were significantly downregulated in silenced lines.