What question did this study set out to answer?

To establish an indirect method for quantitative analysis of intracellular cholesterol and cholesteryl esters via HPLC.

June 4, 2026PeerJOpen Access

Indirect HPLC assay in cellular matrices for cholesterol and cholesteryl esters through oxidation to cholestenone

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Randomized trial establishes an indirect HPLC method for quantifying cholesterol in cellular matrices, highlighting its effectiveness.

To establish an indirect method for quantitative analysis of intracellular cholesterol and cholesteryl esters via HPLC.
Enzymatic hydrolysis of cell lysates at 37 °C for 2 hours
Biphasic lipid extraction with n-hexane/isopropanol
HPLC analysis with isocratic elution and UV detection at 240 nm.
Good linearity within 2.5–160 μg/mL (R² > 0.996)
LOD of 0.05 μg/mL and LOQ of 0.15 μg/mL
Intraday recovery rates ranged from 88.52% to 94.08% with RSDs < 10%.

Li et al. (2026) studied this question.

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