What question did this study set out to answer?

The aim is to develop and validate a rapid detection method for Toxoplasma gondii antibodies applicable across various host species.

June 4, 2026Transboundary and Emerging DiseasesOpen Access

Development and Field Validation of a Double‐Antigen Sandwich Colloidal Gold Immunochromatographic Strip for Detection of Toxoplasma gondii Antibodies in Multiple Host Species

View Full Paper

Ask AI

Bookmark

Authors

XMXin MuNanjing Agricultural University CCChen ChenNanjing Agricultural University XPXianglin PuNanjing Agricultural University

Discussion

Member takes

Implication

Randomized trial evaluates a novel assay for detecting Toxoplasma gondii antibodies in multiple host species, suggesting its utility for rapid diagnostics.

Key Points

The aim is to develop and validate a rapid detection method for Toxoplasma gondii antibodies applicable across various host species.
Developed a double‐antigen sandwich colloidal gold immunochromatographic assay using recombinant surface antigen 2 (rSAG2).
Optimized parameters like conjugation pH, antigen loading, and buffer composition.
Tested 409 clinical serum samples from six animal groups for seropositivity rates and agreement with commercial ELISA kits.
The assay had a detection limit of a 1:40 serum dilution with no cross-reactivity to 23 common pathogens.
Seropositivity rates across animal groups ranged from 2.9% to 31.3%.
The assay showed overall agreement rates of 91.3% for chickens and 97.7% for dogs when compared to commercial ELISA kits.

Cite This Study

Mu et al. (2026) studied this question.

synapsesocial.com/papers/6a2117bfd499ed480b17095c https://doi.org/10.1155/tbed/5879710

View Full Paper

Ask AI

Bookmark

Implication

Randomized trial evaluates a novel assay for detecting Toxoplasma gondii antibodies in multiple host species, suggesting its utility for rapid diagnostics.

Development and Field Validation of a Double‐Antigen Sandwich Colloidal Gold Immunochromatographic Strip for Detection of Toxoplasma gondii Antibodies in Multiple Host Species

Discussion

Member takes

Implication

Key Points

Cite This Study

Development and Field Validation of a Double‐Antigen Sandwich Colloidal Gold Immunochromatographic Strip for Detection of Toxoplasma gondii Antibodies in Multiple Host Species

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider