Key result
Multiple myosin VI molecules can coordinate to efficiently transport vesicle-size cargo over 10 microm of a dense interlaced actin network, with monomers achieving speeds similar to dimers.
Multiple myosin VI molecules, including monomeric forms, can coordinate to efficiently transport cargo across dense F-actin networks.
May inform models of intracellular transport in cardiomyocytes; leaves open whether coordination extends to human cardiac actin networks.
Unconventional myosins interact with the dense cortical actin network during processes such as membrane trafficking, cell migration, and mechanotransduction. Our understanding of unconventional myosin function is derived largely from assays that examine the interaction of a single myosin with a single actin filament. In this study, we have developed a model system to study the interaction between multiple tethered unconventional myosins and a model F-actin cortex, namely the lamellipodium of a migrating fish epidermal keratocyte. Using myosin VI, which moves toward the pointed end of actin filaments, we directly determine the polarity of the extracted keratocyte lamellipodium from the cell periphery to the cell nucleus. We use a combination of experimentation and simulation to demonstrate that multiple myosin VI molecules can coordinate to efficiently transport vesicle-size cargo over 10 microm of the dense interlaced actin network. Furthermore, several molecules of monomeric myosin VI, which are nonprocessive in single molecule assays, can coordinate to transport cargo with similar speeds as dimers.
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Sivaramakrishnan et al. (2009) studied this question. Multiple tethered myosin VI molecules was evaluated on Transport of vesicle-size cargo over the dense interlaced actin network. Multiple myosin VI molecules can coordinate to efficiently transport vesicle-size cargo over 10 microm of a dense interlaced actin network, with monomers achieving speeds similar to dimers.
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