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The aim is to investigate the effects of PDGF on human glomerular mesangial cells, focusing on its proliferation and mRNA expression.

Mesangial cells express PDGF mRNAs and proliferate in response to PDGF

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The aim is to investigate the effects of PDGF on human glomerular mesangial cells, focusing on its proliferation and mRNA expression.
Cultured human glomerular mesangial cells were analyzed for PDGF release and mitogenic activity.
Northern blot analysis was performed to assess PDGF A- and B-chain mRNA expression.
Binding assays were conducted to confirm specific PDGF interaction with mesangial cells.
Mesangial cells secrete a PDGF-like protein that competes for PDGF binding, promoting fibroblast mitogenesis.
Northern blotting demonstrated the presence of both PDGF A- and B-chain mRNAs in mesangial cells.
PDGF binding to mesangial cells resulted in increased DNA synthesis and cell proliferation.

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Investigation shows PDGF stimulates proliferation in glomerular mesangial cells, indicating a growth factor role.

Shultz et al. (1988) studied this question.

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