July 1, 1985Biochemistry

Transient kinetics of ATP hydrolysis by covalently crosslinked actomyosin complex in water and 40% ethylene glycol by the rapid flow quench method

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Population

Covalently cross-linked actomyosin subfragment 1 (acto-SF-1), reversible acto-SF-1, and SF-1

Comparison

Rapid flow quench method with ATP chase or Pi… vs Reversible acto-SF-1 and SF-1 under identical…

Design

Preclinical

Authors

JBJosep A. BioscaUniversitat Autònoma de Barcelona FTFranck TraversInstitut de Recherche en Infectiologie de Montpellier TBT. E. BarmanCentre National de la Recherche Scientifique

Discussion

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Implication

ATP binding kinetics vary with cross-linking; extends biochemical models but leaves open cardiac sarcomere applications.

Structured PICO

Population

Covalently cross-linked actomyosin subfragment 1 (acto-SF-1), reversible acto-SF-1, and SF-1

Intervention

Rapid flow quench method with ATP chase or Pi burst in water and 40% ethylene glycol

Comparator

Reversible acto-SF-1 and SF-1 under identical conditions

Outcome

Kinetics of ATP binding and hydrolysissurrogate

The study characterizes the transient kinetics of ATP hydrolysis by cross-linked actomyosin subfragment 1, showing differences in saturation kinetics and rate constants compared to reversible acto-SF-1 and SF-1.

Cite This Study

Biosca et al. (1985) studied this question.

synapsesocial.com/papers/6a216861cdf8429e7e5fa45e https://doi.org/10.1021/bi00335a059

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Transient kinetics of ATP hydrolysis by covalently crosslinked actomyosin complex in water and 40% ethylene glycol by the rapid flow quench method

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Transient kinetics of ATP hydrolysis by covalently crosslinked actomyosin complex in water and 40% ethylene glycol by the rapid flow quench method

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider