Key result
The ATP-chase method demonstrated a two-step binding process for ATP to myosin subfragment 1, yielding unambiguous values for K1 and k+2 that are sensitive to experimental conditions.
Population
Reaction mixtures containing [gamma-32P]ATP plus myosin subfragment 1
Design
Preclinical
Authors
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No immediate clinical implications; extends kinetic models of myosin-ATP binding for further mechanistic study.
This basic science study provides evidence for a two-step, electrostatically driven binding of ATP to myosin subfragment 1 using the rapid-flow-quench method.
Barman et al. (1983) studied Myosin ATPase mechanism. ATP-chase method was evaluated on K1 and k+2 values for ATP binding. The ATP-chase method demonstrated a two-step binding process for ATP to myosin subfragment 1, yielding unambiguous values for K1 and k+2 that are sensitive to experimental conditions.
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