What question did this study set out to answer?

Determine the kinetics and equilibrium constants associated with ATP synthesis at the myosin S1 active site.

October 1, 1974European Journal of BiochemistryOpen Access

Synthesis of ATP from ADP and Inorganic Phosphate at the Myosin‐Subfragment 1 Active Site

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Population

Myosin S1 fragment

Design

Preclinical

Authors

HMHans Georg MannherzSt. Josef-Hospital HSHelmut SCHENCKMax Planck Society RGRoger S. GoodyMax Planck Institute of Molecular Physiology

Discussion

Member takes

Implication

Biochemical insight into myosin ATP binding; leaves open relevance to cardiac energetics without physiological validation.

Key Points

Determine the kinetics and equilibrium constants associated with ATP synthesis at the myosin S1 active site.
Measured ATP synthesis rates from ADP and inorganic phosphate at 23 °C and pH 8.
Calculated equilibrium and dissociation constants based on experimental data.
The equilibrium constant for ATP synthesis was about 16 at pH 8.
Dissociation constant of inorganic phosphate from the S1 · ADP complex was about 10 mM.
Dissociation constant of ATP was extremely small, approximately 0.10 pM.

Structured PICO

Population

Myosin S1 fragment

Intervention

Synthesis of ATP from ADP and inorganic phosphate

Outcome

Equilibrium constant of the rate limiting step and dissociation constants

The study provides fundamental biochemical insights into the ATPase mechanism of the myosin S1 fragment, revealing an extremely small dissociation constant for ATP.

Cite This Study

Mannherz et al. (1974) studied this question.

synapsesocial.com/papers/6a74c95af5fc394144033682 https://doi.org/10.1111/j.1432-1033.1974.tb03767.x

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Synthesis of ATP from ADP and Inorganic Phosphate at the Myosin‐Subfragment 1 Active Site

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Synthesis of ATP from ADP and Inorganic Phosphate at the Myosin‐Subfragment 1 Active Site

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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