Interaction of Autotaxin With Lipoprotein(a) in Patients With Calcific Aortic Valve Stenosis

Figure 1. Autotaxin Activity and Lysophosphatidic Acid Content in Lipoprotein(a) Versus Low-Density Lipoproteins Purity confirmation by Sebia Hydragel (A), measurement of autotaxin (ATX) activity (B), and lysophoshphatidic acid (LysoPA) content (C) in lipoprotein (a) [Lp(a)] and low-density lipoprotein (LDL) of healthy participants. ATX activity (relative light absorbent unit) and LysoPA content (μM) were normalized by cholesterol content (μmol/l). Asterisk (∗) indicates values significantly different from LDL (p = 0.016 for ATX activity [B] and p = 0.031 for LysoPA content [C]). VLDL = very low density lipoprotein.

Figure 2. Binding Curve of Apo(a) to ATX The binding curve is fitted by a nonlinear model in Prism. Apo(a) = apolipoprotein(a); ATX = autotaxin.

Figure 3. Distribution of ATX-Apo(a) and ATX-ApoB of Study Participants Distribution of ATX-apo(a) (A) and ATX-apoB (B) in patients with (cases) and without (controls) calcific aortic valve stenosis. apoB = apolipoprotein B; other abbreviations as in Figures 1 and 2.

Figure 4. Spearman Correlation Between Plasma Levels of Biomarkers Spearman correlation between plasma levels of ATX-apo(a) and ATX-apoB (A), ATX-apo(a) and Lp(a) (B), ATX-apo(a) and ATX (C) Abbreviations as in Figures 1, 2, and 3.

Figure 5. OR for Aortic Stenosis According to BMI and ATX-Apo(a) Odds ratio (OR) for aortic stenosis (calcific aortic valve stenosis [CAVS]) in patients according to ATX-apo(a) and body mass index (BMI). OR calculated after corrections (same as Model 2). Apo(a) = apolipoprotein(a).