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Seven metabolites were isolated by reversed phase partition chromatography and thin layer chromatography after incubation of tritium-labeled prostaglandin E2 with the soluble fraction of homogenates of guinea pig liver. The two major compounds (forming about 61% of the recovered radioactivity) were identified as 11α,15-dihydroxy-9-ketoprost-5-enoic acid and 11α-hydroxy-9,15-diketoprost-5-enoic acid. Three compounds (together about 26% of the recovered radioactivity) belonged to the F series of prostaglandins, and were identified as prostaglandin F2α, 9α,11α,15-trihydroxyprost-5-enoic acid, and 9α,11α-dihydroxy-15-keto-prost-5-enoic acid. The isolation of these compounds after incubation of prostaglandin E2 for the first time showed that prostaglandin Fα compounds can be formed from prostaglandin E compounds in animal tissue. Two minor compounds (together about 5% of the recovered radioactivity) were identified as 8-isoprostaglandin E2 and, tentatively, 8-isoprostaglandin F2α.
Hámberg et al. (Thu,) studied this question.