The PPCDQ polymeric micelle adjuvant system elicited robust antiviral humoral and cellular immune responses and protected mice from lethal influenza A and rabies virus challenges.
Does the PPCDQ nanovaccine system improve humoral and cellular immune responses in mice compared to soluble proteins?
The PPCDQ polymeric micelle adjuvant system significantly enhances humoral and cellular immunity for recombinant protein antigens, offering a potent nanoplatform for antiviral vaccines.
Although protein subunit vaccines generally have acceptable safety profiles with precise antigenic content, limited immunogenicity can lead to unsatisfactory humoral and cellular immunity and the need for vaccine adjuvants and delivery system. Herein, we assess a vaccine adjuvant system comprising Quillaja Saponaria-21(QS-21) and cobalt porphyrin polymeric micelles that enabling the display of His-tagged antigen on its surface. The nanoscale micelles promote antigen uptake and dendritic cell activation to induce robust cytotoxic T lymphocyte response and germinal center formation. Using the recombinant protein antigens from influenza A and rabies virus, the micelle adjuvant system elicited robust antiviral responses and protected mice from lethal challenge. In addition, this system could be combined with other antigens to induce high titers of neutralizing antibodies in models of three highly pathogenic viral pathogens: Ebola virus, Marburg virus, and Nipah virus. Collectively, our results demonstrate this polymeric micelle adjuvant system can be used as a potent nanoplatform for developing antiviral vaccine countermeasures that promote humoral and cellular immunity.
Wang et al. (Wed,) conducted a other in Viral infection (Influenza A, Rabies, Ebola, Marburg, Nipah). PPCDQ polymeric micelle adjuvant system with viral antigens vs. Soluble protein antigens alone or with commercial adjuvants was evaluated on Lethal viral challenge survival and neutralizing antibody titers. The PPCDQ polymeric micelle adjuvant system elicited robust antiviral humoral and cellular immune responses and protected mice from lethal influenza A and rabies virus challenges.