The number of proteins in rat liver ribosomes was determined by two-dimensional polyacrylamide gel electrophoresis. There were 30 proteins in the 40 S subunit, 39 in the 60 S subunit. The proteins of the two subunits, with one possible exception, are unique. Preparations of 80 S ribosomes contained three proteins not present in either subunit; thus, we estimate that eukaryotic ribosomes contain between 68 and 72 different proteins.
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Sherton et al. (1972) studied this question.
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