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This study investigates the growth and structural polarities of microtubules initiated by centrosomes and chromosomes in vitro.

Polarity of microtubules nucleated by centrosomes and chromosomes of Chinese hamster ovary cells in vitro

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Experimental analysis of microtubule growth in cells reveals orientation changes affecting their structural dynamics.

This study investigates the growth and structural polarities of microtubules initiated by centrosomes and chromosomes in vitro.
Analyzed the kinetics of microtubule growth from centrosomes, chromosomes, and flagellar seeds.
Compared rates of elongation to evaluate the growth dynamics at different ends.
Conducted internal control experiments using flagellar-seeded microtubules.
Centrosomal microtubules elongate exclusively at the plus end with equivalent depolymerization rate (p < 0.05).
Chromosomal microtubules exhibit a similar growth pattern, with plus ends oriented distally.
Structural limitations are indicated by the inactivity of the minus ends in microtubule dynamics.

Bergen et al. (1980) studied this question.

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