Key result
A subcellular system actively replicating Kilham rat virus DNA in vitro demonstrated that DNA polymerase alpha plays a major role in viral DNA synthesis.
Demonstrates the role of DNA polymerase alpha and a cytosolic factor in Kilham rat virus DNA replication using a subcellular in vitro system.
A subcellular system actively replicating Kilham rat virus DNA in vitro was developed. Cellular lysates and isolated nuclei from infected cells showed an amplification of replicative forms in vitro. Solubilized replicative complexes, either partly purified or in the form of a crude extract, were able to synthesize replicative forms and single-stranded DNA. DNA polymerase alpha played a major role in Kilham rat virus DNA synthesis in vivo and in vitro. Furthermore, a factor present in the cytosol of infected cells increased the polymerizing activity of viral replicative complexes.
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Gunther et al. (1984) studied Kilham rat virus infection. Subcellular system was evaluated on Kilham rat virus DNA synthesis. A subcellular system actively replicating Kilham rat virus DNA in vitro demonstrated that DNA polymerase alpha plays a major role in viral DNA synthesis.
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