Key result
Inactivation of the Pelle kinase domain or elimination of the Tube-Pelle interaction dramatically increases Tube recruitment to the ventral plasma membrane in regions of active signaling.
Pelle functions to downregulate the signal-dependent relocalization of Tube, indicating a feedback regulation mechanism in the Drosophila Toll signaling pathway.
No immediate clinical implications; leaves open whether Pelle-mediated feedback extends to mammalian TLR pathways.
Dorsoventral polarity in the Drosophila embryo is established through a signal transduction cascade triggered in ventral and ventrolateral regions. Activation of a transmembrane receptor, Toll, leads to localized recruitment of the adaptor protein Tube and protein kinase Pelle. Signaling through these components directs degradation of the IkappaB-like inhibitor Cactus and nuclear translocation of the Rel protein Dorsal. Here we show through confocal immunofluorescence microscopy that Pelle functions to downregulate the signal-dependent relocalization of Tube. Inactivation of the Pelle kinase domain, or elimination of the Tube-Pelle interaction, dramatically increases Tube recruitment to the ventral plasma membrane in regions of active signaling. We also characterize a large collection of pelle alleles, identifying the molecular lesions in these alleles and their effects on Pelle autophosphorylation, Tube phosphorylation and Tube relocalization. Our results point to a mechanism operating to modulate the domain or duration of signaling downstream from Tube and Pelle.
No takes yet. Share an insight, caveat, or question.
Towb et al. (2001) studied Drosophila embryo development. Inactivation of Pelle kinase domain or elimination of Tube-Pelle interaction vs. Normal signaling was evaluated on Tube recruitment to the ventral plasma membrane. Inactivation of the Pelle kinase domain or elimination of the Tube-Pelle interaction dramatically increases Tube recruitment to the ventral plasma membrane in regions of active signaling.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: