What question did this study set out to answer?

This research aims to understand how L-type calcium channels behave under different voltage and current conditions in embryonic chick heart cells.

November 1, 1991The Journal of PhysiologyOpen Access

Gating of L‐type Ca2+ channels in embryonic chick ventricle cells: dependence on voltage, current and channel density.

Q: What is the clinical evidence from this study?

Study design: Other. Population: Embryonic chick heart ventricle cells. Intervention: Voltage depolarization and ion channel blockers. Primary outcome: L-type Ca2+ channel kinetics (mean open time, conductance, inactivation).

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Key result

L-type calcium channels in embryonic chick heart ventricle exhibit voltage-dependent, time-variant kinetics that depend on channel density and holding potential.

Population

Embryonic chick heart ventricle cells

Comparison

Voltage depolarization and varying holding… vs Different holding potentials and channel densities

Design

Preclinical

Authors

MMMichele MazzantiUniversity of Milano-Bicocca LDLouis J. DeFeliceVirginia Commonwealth University YLY M LiuEmory University

Discussion

Member takes

Implication

Chick embryo data warrant no clinical extrapolation; leaves open species-specific validation of L-type channel kinetics in mammals.

Key Points

This research aims to understand how L-type calcium channels behave under different voltage and current conditions in embryonic chick heart cells.
Investigated voltage-dependent kinetics of single L-type Ca2+ channels using cell-attached patches.
Analyzed whole-cell currents in differing ion conditions and drug treatments.
Examined channel behavior under varying densities and membrane potentials.
Mean open time of L-type Ca2+ channels increases from 1.4 ms to 4.2 ms with voltage from -20 mV to 20 mV (p < 0.05).
Currents from multichannel patches inactivate rapidly, displaying large tail currents when held at -80 mV but slower currents at -40 mV.
Whole-cell currents are completely blocked by 20 µM-nifedipine, confirming the presence of L-type channels.

Structured PICO

Population

Embryonic chick heart ventricle cells

Exposure

Voltage depolarization and varying holding potentials (-80 mV vs -40 mV) in the presence of Ba2+ or Ca2+

Comparator

Different holding potentials (-80 mV vs -40 mV) and channel densities (multichannel vs 1-2 channels)

Outcome

L-type Ca2+ channel gating kinetics (mean open time, single-channel conductance, inactivation)surrogate

In embryonic chick ventricle cells, L-type calcium channel gating kinetics are dependent on voltage, current, and channel density.

Cite This Study

Mazzanti et al. (1991) studied Embryonic chick heart ventricle cells. Voltage depolarization and ion channel blockers was evaluated on L-type Ca2+ channel kinetics (mean open time, conductance, inactivation). L-type calcium channels in embryonic chick heart ventricle exhibit voltage-dependent, time-variant kinetics that depend on channel density and holding potential.

synapsesocial.com/papers/6a21cbf74c1bee377cecaed3 https://doi.org/10.1113/jphysiol.1991.sp018835

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Gating of L‐type Ca2+ channels in embryonic chick ventricle cells: dependence on voltage, current and channel density.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Gating of L‐type Ca2+ channels in embryonic chick ventricle cells: dependence on voltage, current and channel density.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider