Yeast Nuclear Extract Contains Two Major Forms of RNA Polymerase II Mediator Complexes
Authors
Discussion
Loading...
Member takes
Overview
Randomized trial identifies major forms of Mediator complexes in yeast, suggesting roles in transcription activation.
Key Points
- The study aims to identify the major forms of the Mediator complex involved in transcription by RNA polymerase II in yeast.
- Mediator was isolated from nuclear extracts using a two-step chromatographic procedure.
- Ion exchange chromatography and high salt conditions were avoided to ensure subunit stability.
- Components of the Mediator complexes were identified using mass spectrometry and Western analysis.
- The major Mediator form identified as pol II x Med contained RNA polymerase II and the Srb8-11 module.
- A second complex, Mediator core (Medc), was identified, lacking several key components including pol II and Srb8-11.
- The pol II x Med complex was more active in transcription than the Medc complex, which had significantly lower activity.
Cite This Study
Liu et al. (2001) studied this question.