Assessment of the Reversion to Virulence and Protective Efficacy in Pigs Receiving the Live Attenuated Classical Swine Fever Recombinant Vaccine Candidate FlagT4G

Figures

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  Figure 1. Evolution of body temperature in animals inoculated with FlagT4G during five consecutive passages (panels Passage 1–5, respectively). Data are presented as individual values (expressed as °C) for each of the inoculated animals in each of the different groups. The color lines represent the data of each animal.

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  Figure 2. Antigenic profile of FlagT4G virus after the consecutive passages in the reversion to virulence experiment (FlagT4Gv p5). Reactivity of the FlagT4Gv p5 with monoclonal antibody specific for Flag and (Anti-Flag mAb) and CSFV E2 epitope recognized by monoclonal antibody WH303 (WH303 mAb) compared with the stock FlagT4G virus (FlagT4Gv) and BICv.

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  Figure 3. Kinetics of body temperature values in pigs intramuscularly inoculated with different doses 102–105 TCID50) of FlagT4G virus or Mock Vaccinated before (Days post-inoculation) and after intranasal challenge (Days post-challenge) with 105 TCID50 of the virulent parental virus BICv. Each curve represents individual data from each of the animals under each of the treatments. The color lines represent the data of each group.

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  Figure 4. Evolution of mortality in pigs intramuscularly inoculated with different doses 102–105 TCID50) of FlagT4G virus or Mock Vaccinated and challenged with 105 TCID50 of the virulent parental virus BICv.