The fluorescence decay of aqueous tryptophan is shown to be described by a two exponential decay function whose components have life-times of 3.14 ns and 0.51 ns. These components are assigned as the solvent equilibrated 1 L a and 1 L b , states respectively. The time-resolved emission spectra are presented and can be resolved into two spectra with λ max at 350 nm and 335 nm corresponding to these two states.
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Rayner et al. (1978) studied this question.
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