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Under anaerobic incubations, myocytes excluded Trypan blue despite severe derangements in biochemical functions, suggesting Trypan blue entry is an insensitive criterion of cell injury.
Trypan blue exclusion is an insensitive marker of isolated myocyte viability; a combination of rod-shape morphology, ATP levels, and contractility under pacing is recommended instead.
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Trypan blue exclusion may overestimate myocyte viability in ischemia models; leaves open validation of multimodal criteria for experimental research.
Cheung et al. (1984) studied Isolated cardiac myocyte viability. Anaerobic incubations (absence of extracellular calcium or presence of 6% PEG) vs. Aerobic incubation was evaluated on Trypan blue exclusion vs biochemical functions. Under anaerobic incubations, myocytes excluded Trypan blue despite severe derangements in biochemical functions, suggesting Trypan blue entry is an insensitive criterion of cell injury.
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