Parasagittal organization of the rat cerebellar cortex: Direct comparison of purkinje cell compartments and the organization of the spinocerebellar projection

Retrograde and anterograde transport of tracers, electrophysiological recording, somatotopic mapping, and histochemical and immunological techniques have all revealed a parasagittal parcellation of the cerebellar cortex, including its efferent and many of its afferent connections. In order to establish whether the different compartments share a common organizational plan, a systematic comparative analysis of the patterns of parasagittal zonation in the cerebellar cortex of the rat has been undertaken, by using the parasagittal compartmentation of zebrin I+ and zebrin I- Purkinje cells as revealed by monoclonal antibody Q113 as a reference frame. The distribution of mossy fiber terminals originating from the lower thoracic-higher lumbar spinal cord was compared to the distribution of zebrin I bands. Three-dimensional reconstructions from alternate frontal sections processed either for the anterograde transport of tracer or for zebrin I immunoreactivity reveal that the limits of the spinocerebellar terminal fields in the granular layer correlate well with the boundaries of some, but not all, zebrin I compartments in the molecular layer above. This leads to a subdivision of the zebrin I compartments into spinal receiving and spinal nonreceiving portions. In lobules II and VIII, the spinocerebellar terminal fields assume different positions relative to the zebrin I compartments in the ventral compared to the dorsal faces. Thus, each longitudinal compartment may be further divided transversely into subzones, each receiving a specific combination of mossy fiber afferents. The further subdivision of zebrin I compartments by mossy fiber terminal fields increases the resolution of the topography to such a point that anatomical compartment widths become compatible with the width of the microzones and the patches identified by electrophysiological methods.